国产大片91精品免费观看3,精品国产99久久久久久,18禁精品久久久国产精品久,玖玖玖国产精品一区二区,亚洲五月婷婷综合网,亚洲中文av字幕在线观看</title>

探究腫瘤細胞的起源及演化對理解癌癥的發(fā)生、復(fù)發(fā)以及對后續(xù)腫瘤的預(yù)防和治療至關(guān)重要。腫瘤細胞起源于正常細胞內(nèi)突變的積累，然后在微環(huán)境、手術(shù)、放化療等不同的選擇壓力下，經(jīng)歷多個階段的演化。對演化過程的研究能夠揭示癌癥的發(fā)生、發(fā)展規(guī)律，鑒定癌細胞起源及演化相關(guān)的基因組事件，進而為治療腫瘤提供新的藥物靶點。研究組前期通過開發(fā)高通量單細胞DNA測序技術(shù)（Arc-well）、單細胞進行空間位置（或樣本信息）標記的方法SNuBar以及多種基于高通量測序的低頻突變檢測方法（o2n-seq, Droplet-CirSeq, Oseq和EasyMF）系統(tǒng)研究了乳腺癌原發(fā)到復(fù)發(fā)的克隆演化關(guān)系，揭示了原發(fā)到復(fù)發(fā)癌的克隆演化模型，鑒定了與乳腺癌復(fù)發(fā)相關(guān)的基因組變異事件，為后續(xù)乳腺癌的治療提供了目標靶點。課題組將繼續(xù)通過開發(fā)高通量單細胞多組學測序技術(shù)、空間組學測序技術(shù)等來回答關(guān)于腫瘤細胞起源和演化的問題：如體細胞突變?nèi)绾螌е履[瘤的發(fā)生，導致腫瘤起源的上皮細胞亞型，腫瘤起始細胞空間定位、多組學表征及其與微環(huán)境互作關(guān)系，衰老與腫瘤的關(guān)系等。

1. Wang K^*#, Ye R^*, Bai S, Xiao Z, Yang L, Li J, Tang C, Sei E, Peng J, Casasent AK, Lin SH, Nagi C, Thompson AM, Krishnamurthy S, Navin N^#. Coalescing single-cell genomes and transcriptomes to decode breast cancer progression. Cell. 2025. Online. doi: 10.1016/j.cell.2025.08.012
2. Wang K^*, Kumar T^*, Wang J, Minussi, D, Sei E, Li J, Tran T, Thennavan A, Hu M, Casasent A, Xiao Z, Bai S, King L, Shah V, Kristel P, Borden C, Marks J, Zhao Y, Zurita A, Aparicio A, Chapin B, Ye J, Zhang J, Gibbons D, Grand Challenge PRECISION Consortium, Sawyer E, Thompson A, Futreal A, Hwang ES, Wesseling J, Lips E, Navin N^#. Archival single cell genome sequencing reveals persistent subclones over years to decades of DCIS progression. Cell. 2023; 186(18):3968-3982.e15. doi: 10.1016/j.cell.2023.07.024
3. Wang K^#. Revealing genomic secrets of archival FFPE samples. Nature Reviews Cancer. 2024. doi: 10.1038/s41568-024-00686-7.
4. Wang K^*, Xiao Z^*, Yan Y^*, Ye R, Hu M, Bai S, Sei E, Qiao Y, Chen H, Lim B, Lin S, Navin N^#. Highly-multiplexed sample and spatial barcoding of single cell chromatin accessibility using a single oligonucleotide. Molecular Cell. 2021; 81, 4319-4332. doi:10.1016/j.molcel.2021.09.026.
5. Wang K, Lai S, Chang Y, Lu X, Wu C^#, Ruan J^#. Ultra-precisely and low bias screen low frequency mutations by o2n-seq. Nature communications. 2017; 8:15335. doi: 10.1038/ncomms15335.
6. Wang K^*, Ma X^*, Zhang X, Wu D, Sun C, Sun Y, Lu X, Wu C^#, Guo C^#, Ruan J^#. Using ultra-sensitive next generation sequencing to dissect DNA damage-induced mutagenesis. Scientific Reports. 2016; 6:25310. doi: 10.1038/srep25310.
7. Wang K, Ma Q, Jiang L, Lai S, Lu X, Hou Y^#, Wu C^#, Ruan J^#. Ultra-precise detection of mutations by droplet-based amplification of circularized DNA. BMC Genomics. 2016; 17:214. doi: 10.1186/s12864-016-2480-1.
8. Lin Y^*, Wang J^*, Wang K, Bai S, Thennavan A, Wei R, Yan Y, Li J, Elgamal H, Sei E, Casasent A, Rao M, Tang C, Multani AS, Ma J, Montalvan J, Nagi C, Winocour S, Lim B, Thompson A, Navin N^#. Normal breast tissues harbour rare populations of aneuploid epithelial cells. Nature. 2024 Dec;636(8043):663-670. doi: 10.1038/s41586-024-08129-x.
9. Minussi D^*, Nicholson M^*, Ye H^*, Davis A, Wang K, Baker T, Sei E, Du H, Rabbani M, Peng C, Hu M, Bai S, Lin Y, Schalck A, Multani A, Ma J, McDonald T, Tarabichi M, Casasent A, Barrera A, Chen H, Lim B, Arun B, Meric-Bernstam F, Loo P, Michor F^#, Navin N^#. Breast Tumors Maintain a Reservoir of Subclonal Diversity During Primary Expansion. Nature. 2021. doi: 10.1038/s41586-021-03357-x.
10. Yu L^*#, Wang G^*, Ruan J^*, Chen Y^*, Yang C^*, Cao X^*, Wu H^*, Liu Y^*, Du Z^*, Wang X^*, Yang J^*, Cheng S^*, Zhong L, Wang L, Wang X, Hu J, Fang L, Bai B, Wang K, Yuan N, Wu S, Li B, Zhang J, Yang Y, Zhang C, Long Y, Li H, Yang J, Irwin D, Ryder O, Li Y, Wu C^#, Zhang Y^#. Genomic analysis of snub-nosed monkeys (Rhinopithecus) identifies genes and processes related to high-altitude adaptation. Nature Genetics. 2016; 48, 947–952. doi: 10.1038/ng.3615.